主要原著論文（冨塚）

Rapid human genomic DNA cloning into mouse artificial chromosome via direct chromosome transfer from human iPSC and CRISPR/Cas9-mediated translocation.Miyamoto H, Kobayashi H, Kishima N, Yamazaki K, Hamamichi S, Uno N, Abe S, Hiramuki Y, Kazuki K,Tomizuka K, Kazuki Y
Nucleic Acids Res.2024 Feb 9;52(3):1498-1511.[PUBMED]

R-Spondin1 protects gastric stem cells and mitigates gastric GVHD in allogeneic hematopoietic stem cell transplantation.
Hayase E, Ara T, Saito Y, Takahashi S, Yoshioka K, Ohigashi H, Ogasawara R, Yokoyama E, Yamakawa T, Ebata K, Hasegawa Y,Tomizuka K, Teshima T.
Blood Adv.2024 Feb 13;8(3):725-731.[PUBMED]

Treatment of CHO cells with Taxol and reversine improves micronucleation and microcell-mediated chromosome transfer efficiency.Uno N, Satofuka H, Miyamoto H, Honma K, Suzuki T, Yamazaki K, Ito R, Moriwaki T, Hamamichi S,Tomizuka K, Oshimura M, Kazuki Y.
Mol Ther Nucleic Acids.2023 Jul 15;33:391-403.[PUBMED]

Full-length human dystrophin on human artificial chromosome compensates for mouse dystrophin deficiency in a Duchenne muscular dystrophy mouse model.Hiramuki Y, Abe S, Uno N, Kazuki K, Takata S, Miyamoto H, Takayama H, Morimoto K, Takehara S, Osaki M, Tanihata J, Takeda S,Tomizuka K, Oshimura M, Kazuki Y.
Scientific Reports2023 Mar 16;13(1):4360.[PUBMED]

Simultaneous loading of PCR-based multiple fragments on mouse artificial chromosome vectors in DT40 cell for gene delivery. Yamazaki K, Matsuo K, Okada A, Uno N , Suzuki T, Abe S, Hamamichi S, Kishima N, Togai S,Tomizuka K, Kazuki Y.
Scientific Reports2022 Dec 16;12(1):21790.[PUBMED]

Targeted delivery to macrophages and dendritic cells by chemically modified mannose ligand-conjugated siRNA. Keiji Uehara, Toshimasa Harumoto, Asana Makino, Yasuo Koda, Junko Iwano, Yasuhiro Suzuki, Mari Tanigawa, Hiroto Iwai, Kana Asano, Kana Kurihara, Akinori Hamaguchi, Hiroshi Kodaira, Toshiyuki Atsumi, Yoji Yamada,Kazuma Tomizuka.
Nucleic Acids Res.2022 50(9):4840-4859.[PUBMED]

Efficient human-like antibody repertoire and hybridoma production in trans-chromosomic mice carrying megabase-sized human immunoglobulin loci. Hiroyuki Satofuka, Satoshi Abe, Takashi Moriwaki, Akane Okada, Kanako Kazuki, Hiroshi Tanaka, Kyotaro Yamazaki, Genki Hichiwa, Kayoko Morimoto, Haruka Takayama, Yuji Nakayama, Shinya Hatano, Yutaro Yada, Yasufumi Murakami, Yoshihiro Baba, Mitsuo Oshimura,Kazuma Tomizuka, and Yasuhiro Kazuki.
Nature commun.2022 13(1):1841.[PUBMED]

Panel of human cell lines with human/mouse artificial chromosomes. Narumi Uno, Shuta Takata, Shinya Komoto, Hitomaru Miyamoto,Yuji Nakayama, Mitsuhiko Osaki, Ryota Mayuzumi, Natsumi Miyazaki, Chiaki Hando, Satoshi Abe, Tetsushi Sakuma, TakashiYamamoto, Teruhiko Suzuki, Yoshihiro Nakajima, Mitsuo Oshimura,Kazuma Tomizuka, Yasuhiro Kazuki.
Scientific Reports.2022 12(1):3009.[PUBMED]

Engineering of human induced pluripotent stem cells via human artificial chromosome
vectors for cell therapy and disease modeling. Yasuhiro Kazuki, Narumi Uno, Satoshi Abe, Naoyo Kajitani, Kanako Kazuki, Yuwna Yakura, Chiaki Sawada, Shuta Takata, Masaki Sugawara, Yuichi Nagashima, Akane Okada, Masaharu Hiratsuka, Mitsuhiko Osaki, Giulia Ferrari, Francesco Saverio Tedesco, Satoshi Nishikawa, Ken Fukumoto, Shin-ichiro Takayanagi, Atsushi Kunisato, Shin Kaneko, Mitsuo Oshimura andKazuma Tomizuka.
Mol Ther Nucleic Acids.2020 23:629-639[PUBMED]

Development and evaluation of a novel antibody-photon absorber conjugate reveals the possibility of photoimmunotherapy-induced vascular occlusion during treatment in vivo.
Isoda Y, Piao W, Taguchi E, Iwano J, Takaoka S, Uchida A, Yoshikawa K, Enokizono J, Arakawa E,Tomizuka K, Shiraishi Y, Masuda K.
Oncotarget.2018 9(59):31422-31431.[PUBMED]

R-Spondin1 expands Paneth cells and prevents dysbiosis induced by graft-versus-host disease.
Hayase E, Hashimoto D, Nakamura K, Noizat C, Ogasawara R, Takahashi S, Ohigashi H, Yokoi Y, Sugimoto R, Matsuoka S, Ara T, Yokoyama E, Yamakawa T, Ebata K, Kondo T, Hiramine R, Aizawa T, Ogura Y, Hayashi T, Mori H, Kurokawa K,Tomizuka K, Ayabe T, Teshima T.
J Exp Med.2017 Dec 4;214(12):3507-3518.[PUBMED]

Novel anticarcinoembryonic antigen antibody-drug conjugate has antitumor activity in the existence of soluble antigen.
Shinmi D, Nakano R, Mitamura K, Suzuki-Imaizumi M, Iwano J, Isoda Y, Enokizono J, Shiraishi Y, Arakawa E,Tomizuka K, Masuda K.
Cancer Med.2017 Apr;6(4):798-808.[PUBMED]

The soluble form of BMPRIB is a novel therapeutic candidate for treating bone related disorders.
Yamawaki K, Kondo Y, Okada T, Oshima T, Kakitani M,Tomizuka K.
Sci Rep.2016 Jan 6;6:18849.[PUBMED]

Trans-chromosomic mice containing a human CYP3A cluster for prediction of xenobiotic metabolism in humans.
Kazuki Y, Kobayashi K, Aueviriyavit S, Oshima T, Kuroiwa Y, Tsukazaki Y, Senda N, Kawakami H, Ohtsuki S, Abe S, Takiguchi M, Hoshiya H, Kajitani N, Takehara S, Kubo K, Terasaki T, Chiba K,Tomizuka K, Oshimura M.
Hum Mol Genet.2013 Feb 1;22(3):578-92.[PUBMED]

R-spondin1 plays an essential role in ovarian development through positively regulating Wnt-4 signaling.Tomizuka K, Horikoshi K, Kitada R, Sugawara Y, Iba Y, Kojima A, Yoshitome A, Yamawaki K, Amagai M, Inoue A, Oshima T, Kakitani M.
Hum Mol Genet.2008 17(9):1278-91.[PUBMED]

Mitogenic influence of human R-spondin1 on the intestinal epithelium.
Kim KA, Kakitani M, Zhao J, Oshima T, Tang T, Binnerts M, Liu Y, Boyle B, Park E, Emtage P, Funk WD,Tomizuka K.
Science.2005 309(5738):1256-59.[PUBMED]

Manipulation of human minichromosomes to carry greater than megabase-sized chromosome inserts.
Kuroiwa Y,Tomizuka K, Shinohara T, Kazuki Y, Yoshida H, Ohguma A, Yamamoto T, Tanaka S, Oshimura M, Ishida I.
Nat Biotechnol.2000 18(10):1086-90.[PUBMED]

Double trans-chromosomic mice: maintenance of two individual human chromosome fragments containing Ig heavy and kappa loci and expression of fully human antibodies.
Tomizuka K, Shinohara T, Yoshida H, Uejima H, Ohguma A, Tanaka S, Sato K, Oshimura M, Ishida I.
Proc Natl Acad Sci USA.2000 97(2):722-27.[PUBMED]

Functional expression and germline transmission of a human chromosome fragment in chimaeric mice.
Tomizuka K, Yoshida H, Uejima H, Kugoh H, Sato K, Ohguma A, Hayasaka M, Hanaoka K,OshimuraM, Ishida I.
Nat Genet.1997 16(2):133-43.[PUBMED]

 

主要原著論文（横堀）

Hydroxylation of a conserved tRNA modification contributes to establishment of non-universal genetic code in echinoderm mitochondria.
Nagao A, Ohara M, Miyauchi K,Yokobori S, Yamagishi A, Watanabe K, Suzuki T.
Nat Struct Mol Biol. 2017 4(9):778-782.[PUBMED]

Quest for ancestors of eukaryal cells based on phylogenetic analyses of aminoacyl tRNA synthetases.
Furukawa R, Nakagawa M, Kuroyanagi T,Yokobori S, Yamagishi A.
J Mol Evol.(2017) 84: 51-66.[PUBMED]

The possible interplanetary migration of aggregated microbes: Viability of Deinococcus spp. under the environmental conditions at ISS for exposure experiment of microbes in Tanpopo mission.
Kawaguchi Y, Yang Y, Shiraishi K, Kawashiri N, Takahashi Y, Sugino T, Nakagawa K, Tanigawa A, Hashimoto H, Yoshida S, Kobayashi K, Satoh K, Narumi I,Yokobori S, Yamagishi A.
Orig Life Evol Biosph.2013 43 (4-5): 411-428.[PUBMED]

Experimental evidence for the thermophilicity of ancestral life.
Akanuma S, Nakajima Y,Yokobori S, Nemoto N, Mase T, Miyazono K, Kimura M, Tanokura M, Yamagishi A.
Proc Natl Acad Sci USA.2013 110 (27): 11067-11072.[PUBMED]

Life without tRNAArg-adenosine deaminase TadA: evolutionary consequences of decoding the four CGN codons as arginine in Mycoplasmas and other Mollicutes.
Yokobori S, Kitamura A, Grosjean H, Bessho Y.
Nucleic Acids Res.2013 41: 6531-6543.[PUBMED]

Deinococcus aerius sp. nov., isolated from the high atmosphere.
Yang Y, Itoh T,Yokobori S, Itahashi S, Shimada H, Satoh K, Ohba H, INarumi, Yamagishi A.
Int J Syst Evol Microbiol.2009 59 (8): 1862-1866.[PUBMED]

Multiple origins of the ascidian-Prochloron symbiosis: Molecular phylogeny of photosymbiotic and non-symbiotic colonial ascidians inferred from 18S rDNA sequences.
Yokobori S, Kurabayashi A, Neilan BA, Maruyama T, Hirose E.
Mol Phylogenet Evol.2006 40 (1): 8-19.[PUBMED]

Designing thermostable proteins: Ancestral mutants of 3-isopropylmalate dehydrogenase designed by using a phylogenetic tree.
Watanabe K, Ohkuri T,Yokobori S, Yamagishi A.
J Mol Biol.2006 355 (4): 664-674.[PUBMED]

Evolution of pulmonate gastropod mitochondrial genomes: comparisons of gene organizations of Euhadra, Cepaea and Albinaria and implications of unusual tRNA secondary structures.
Yamazaki N, Ueshima R, Terrett J A,Yokobori S, Kaifu M., Segawa A, Kobayashi T, Numachi K, Ueda T, Nishikawa K, Watanabe K, Thomas RH.
Genetics.1007 145 (3): 749-758.[PUBMED]

Transfer RNA editing in land snail mitochondria.
Yokobori S, Pääbo S.
Proc Natl Acad Sci USA.1995 92 (22): 10432-10435.[PUBMED]

 

主要原著論文（宇野）

Rapid human genomic DNA cloning into mouse artificial chromosome via direct chromosome transfer from human iPSC and CRISPR/Cas9-mediated translocation.
Miyamoto H, Kobayashi H, Kishima N, Yamazaki K, Hamamichi S,Uno N, Abe S, Hiramuki Y, Kazuki K, Tomizuka K, Kazuki Y.
Nucleic Acids Res.2024 Feb 9;52(3):1498-1511.[PUBMED]

Treatment of CHO cells with Taxol and reversine improves micronucleation and microcell-mediated chromosome transfer efficiency.
Uno N, Satofuka H, Miyamoto H, Honma K, Suzuki T, Yamazaki K, Ito R, Moriwaki T, Hamamichi S, Tomizuka K, Oshimura M, Kazuki Y.
Mol Ther Nucleic Acids.2023 Jul 15;33:391-403.[PUBMED]

Full-length human dystrophin on human artificial chromosome compensates for mouse dystrophin deficiency in a Duchenne muscular dystrophy mouse model.
Hiramuki Y, Abe S,Uno N, Kazuki K, Takata S, Miyamoto H, Takayama H, Morimoto K, Takehara S, Osaki M, Tanihata J, Takeda S, Tomizuka K, Oshimura M, Kazuki Y.
Sci Rep.2023 Mar 16;13(1):4360.[PUBMED]

Simultaneous loading of PCR-based multiple fragments on mouse artificial chromosome vectors in DT40 cell for gene delivery.
Yamazaki K, Matsuo K, Okada A,Uno N, Suzuki T, Abe S, Hamamichi S, Kishima N, Togai S, Tomizuka K, Kazuki Y.
Sci Rep.2022 Dec 16;12(1):21790.[PUBMED]

Panel of human cell lines with human/mouse artificial chromosomes.
Uno N, Takata S, Komoto S, Miyamoto H, Nakayama Y, Osaki M, Mayuzumi R, Miyazaki N, Hando C, Abe S, Sakuma T, Yamamoto T, Suzuki T, Nakajima Y, Oshimura M, Tomizuka K, Kazuki Y.
Sci Rep.2022 Feb 22;12(1):3009.[PUBMED]

MTA1, a metastasis‑associated protein, in endothelial cells is an essential molecule for angiogenesis.
Ishikawa M, Osaki M,Uno N, Ohira T, Kugoh H, Okada F.
Mol Med Rep.2022 Jan;25(1):11.[PUBMED]

Engineering of human induced pluripotent stem cells via human artificial chromosome vectors for cell therapy and disease modeling.
Kazuki Y,Uno N, Abe S, Kajitani N, Kazuki K, Yakura Y, Sawada C, Takata S, Sugawara M, Nagashima Y, Okada A, Hiratsuka M, Osaki M, Ferrari G, Tedesco FS, Nishikawa S, Fukumoto K, Takayanagi SI, Kunisato A, Kaneko S, Oshimura M, Tomizuka K.
Mol Ther Nucleic Acids.2020 Dec 19;23:629-639.[PUBMED]

A non-mosaic transchromosomic mouse model of down syndrome carrying the long arm of human chromosome 21.
Kazuki Y, Gao FJ, Li Y, Moyer AJ, Devenney B, Hiramatsu K, Miyagawa-Tomita S, Abe S, Kazuki K, Kajitani N,Uno N, Takehara S, Takiguchi M, Yamakawa M, Hasegawa A, Shimizu R, Matsukura S, Noda N, Ogonuki N, Inoue K, Matoba S, Ogura A, Florea LD, Savonenko A, Xiao M, Wu D, Batista DA, Yang J, Qiu Z, Singh N, Richtsmeier JT, Takeuchi T, Oshimura M, Reeves RH.
Elife.2020 Jun 29;9:e56223.[PUBMED]

An efficient protein production system via gene amplification on a human artificial chromosome and the chromosome transfer to CHO cells.
Ohira T, Miyauchi K,Uno N, Shimizu N, Kazuki Y, Oshimura M, Kugoh H.
Sci Rep.2019Nov 18;9(1):16954.[PUBMED]

A luciferase complementation assay system using transferable mouse artificial chromosomes to monitor protein-protein interactions mediated by G protein-coupled receptors.
Uno N, Fujimoto T, Komoto S, Kurosawa G, Sawa M, Suzuki T, Kazuki Y, Oshimura M.
Cytotechnology.2018 70(6):1499-1508.[PUBMED]

Reversible immortalisation enables genetic correction of human muscle progenitors and engineering of next-generation human artificial chromosomes for Duchenne muscular dystrophy.
Benedetti S,Uno N, Hoshiya H, Ragazzi M, Ferrari G, Kazuki Y, Moyle LA, Tonlorenzi R, Lombardo A, Chaouch S, Mouly V, Moore M, Popplewell L, Kazuki K, Katoh M, Naldini L, Dickson G, Messina G, Oshimura M, Cossu G, Tedesco FS.
EMBO Mol Med.2018 10(2):254-275.[PUBMED]

Combinations of chromosome transfer and genome editing for the development of cell/animal models of human disease and humanized animal models.
Uno N, Abe S, Oshimura M, Kazuki Y.
J Hum Genet.2018 Feb;63(2):145-156. Review.[PUBMED]

CRISPR/Cas9-induced transgene insertion and telomere-associated truncation of a single human chromosome for chromosome engineering in CHO and A9 cells.
Uno N, Hiramatsu K, Uno K, Komoto S, Kazuki Y, Oshimura M.
Sci Rep.2017 Oct 6;7(1):12739.[PUBMED]

Dynamics of epigenetic regulation at the single-cell level.
Bintu L, Yong J, Antebi YE, McCue K, Kazuki Y,Uno N, Oshimura M, Elowitz MB.
Science.2016 351(6274):720-4.[PUBMED]

Development of a Safeguard System Using an Episomal Mammalian Artificial Chromosome for Gene and Cell Therapy.
Uno N, Uno K, Komoto S, Suzuki T, Hiratsuka M, Osaki M, Kazuki Y, Oshimura M.
Mol Ther Nucleic Acids.2015 4:e272.[PUBMED]