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Keywords
Microbiology; Thermus thermophilus, DNA repair, Mutagenesis;
Daphnia magna, Genome, Development, Hemoglobin, microRNA

Current research themes in the Laboratory of Environmental Molecular Biology


Gene expression during embryogenesis and gameto- genesis in the water flea, Daphnia magna. Evolution of Hox and carapace developing genes in the water flea, Daphnia.  Oxidative DNA damages and DNA repair in the extremely thermophilic bacterium, Thermus thermophilus
single-minded homolog (green) in midline cells formation RNAi (RNA interference method) Colony analyzer

STAFF
Professor
Toshihiro OHTA, Ph.D.
Associate Professor
Shin-ichi TOKISHITA, Ph.D.
Assistant Professor
Yasuhiro SHIGA, Ph.D.
 ohta (at) toyaku.ac.jp  shinichi (at) toyaku.ac.jp  yshiga (at) toyaku.ac.jp

Publication List (2006-2013)

1.  Ito, K., R. Ishigamori, M. Mutoh, T. Ohta, T. Imai, and M. Takahashi,

Ay allele promotes azoxymethane-induced colorectal carcinogenesis by macrophage migration in hyperlipidemic/diabetic KK mice.

Cancer Science, 104, 835-843 (2013)

 

2.  Onodera, T., K. Satoh, T. Ohta, and I. Narumi,

Deinococcus radiodurans YgjD and YeaZ are involved in the repair of DNA cross- links.

Extremophiles, 17, 171-179 (2013)

 

3.  Sassa, A., N. Kamoshita, T. Matsuda, I. Kuraoka, T. Ohta, T. Nohmi, M. Honma, and M. Yasui,

Miscoding properties of 8-chloro-2’-deoxyguanosine, hypochlorous acid-induced DNA adduct, catalyzed by human DNA polymerases.

Mutagenesis, 28, 81-88 (2013)

 

4. Toyoda-Hokaiwado, N., Y. Yasui, M. Takamune, M. Yamada, M. Muramatsu, K. Masumura, T. Ohta, T. Tanaka, and T. Nohmi,

Opposite modulating effects of capsaicin on N-diethylnitrosamine (DEN)-induced mutagenesis in Salmonella typhimurium YG7108 and DEN-induced hepatocarcinogenesis in gpt delta transgenic rats.

Genes Environ., 33, 160-166 (2011)

 

5.  Toyoda-Hokaiwado, N., Y. Yasui, M. Muramatsu, K. Masumura, M. Takamune, M. Yamada, T. Ohta, T. Tanaka, and T. Nohmi,

Chemopreventive effects of silymarin against 1,2-dimethylhydrazene plus dextran sodium sulfate- induced inflammation-associated carcinogenicity and genotoxicitiy in the colon of gpt delta rats.

Carcinogenesis, 32, 1512-1517 (2011)

 

6.  Abe, H., A. Sato, S. Tokishita, T. Ohta, and H. Ito,

Synthesis of fluorescence pyriproxyfen analogues as juvenile hormone agonist.

Heterocycles, 83, 1649-1658 (2011)

 

7.  Morita, R., H. Hishinuma, H. Ohyama, R. Mega, T. Ohta, N. Nakagawa, Y. Agari, K. Fukui, A. Shinkai, S. Kuramitsu, and R. Masui,

An alkyltransferase-like protein from Thermus thermophilus HB8 affects the regulation of gene expression in alkylation response.

J. Biochem., 150, 327-339 (2011)

 

8.  Sassa, A., T. Ohta, T. Nohmi, M. Honma, and M. Yasui,

Mutational specificities of brominated DNA adducts catalyzed by human DNA polymerases.

J. Mol. Biol., 406, 679-686 (2011)

 

9.  Sassa, A., N. Niimi, H. Fujimoto, A. Katafuchi, P. Grúz, M. Yasui, R. C. Gupta, F. Johnson, T. Ohta, and T. Nohmi,

Phenylalanine 171 is a molecular brake for translesion synthesis across benzo[a]pyrene-guanine adducts by human DNA polymerase kappa.

Mutation Res., 718, 10-17 (2011)

 

10. Onodera, T., K. Morino, S. Tokishita, R. Morita, R. Masui, S. Kuramitsu, and T. Ohta,

Role of alkyltransferase-like (ATL) protein in repair of methylated DNA lesions in Thermusthermophilus.Mutagenesis, 26, 303-308 (2011)

 

11. Colbourne, JK., Pfrender, ME., Gilbert, D., Thomas, WK., Tucker, A., Oakley, TH., Tokishita, S., Aerts, A., Arnold, GJ., Basu, MK., Bauer, DJ., Cáceres, CE., Carmel, L., Casola, C., Choi, JH., Detter, JC., Dong, Q., Dusheyko, S., Eads, BD., Fröhlich, T., Geiler-Samerotte, KA., Gerlach, D., Hatcher, P., Jogdeo, S., Krijgsveld, J., Kriventseva, EV., Kültz, D., Laforsch, C., Lindquist, E., Lopez, J., Manak, JR., Muller, J., Pangilinan, J., Patwardhan, RP., Pitluck, S., Pritham, EJ., Rechtsteiner, A., Rho, M., Rogozin, IB., Sakarya, O., Salamov, A., Schaack, S., Shapiro, H., Shiga, Y., Skalitzky, C., Smith, Z., Souvorov, A., Sung, W., Tang, Z., Tsuchiya, D., Tu, H., Vos, H., Wang, M., Wolf, YI., Yamagata, H., Yamada, T., Ye Y., Shaw, JR., Andrews, J., Crease, TJ., Tang, H., Lucas, SM., Robertson, HM., Bork, P., Koonin, EV., Zdobnov, EM., Grigoriev, IV., Lynch, M., and Boore, JL.,

The Ecoresponsive Genome of Daphnia pulex.

Science, 331, 555-561 (2011).

 

12. Kato, Y., Shiga, Y., Kobayashi, K., Tokishita, S., Yamagata, H., Iguchi, T., and Watanabe, H.,

Development of an RNA interference method in the cladoceran crustacean Daphnia magna.

Dev. Genes Evol., 220, 337-345 (2011)

 

13. Higashiura, Y., Yamaguchi, H., Ishihara, M., Ono, N., Tsukagoshi, H., Yokobori, S., Tokishita, S., Yamagata, H., and Fukatsu, T.,

Male death resulting from hybridization between subspecies of the gypsy moth, Lymantria dispar.

Heredity, 106, 603-613 (2011).

 

14. Koshida, H., T. Onodera, R. Arai, Y. Inaba, S. Uchiyama, and T. Ohta,

Photomutagenicity of sidestream smoke condensate of cigarette with UVA-irradiation.

Genes Environ., 32, 25-30 (2010)

 

15. Katafuchi, A., A. Sassa, N. Niimi, P. Gruz, H. Fujimoto, C. Masutani, F. Hanaoka, T. Ohta, and T. Nohmi,

Critical amino acids in human DNA polymerases η and κ involved in erroneous incorporation of oxidized nucleotides.

Nucleic Acids Res., 38, 859-867 (2010)

 

16. Niimi, N., A. Sassa, A. Katafuchi, P. Gruz, H. Fujimoto, R. Bonala, F. Johnson, T. Ohta, and T. Nohmi,

The steric gate amino acid tyrosine 112 is required for efficient mismatched-primer extension by human DNA polymeraseκ.

Biochemistry, 48, 4239-4246 (2009)

 

17. Sakai, T., S. Tokishita, K. Mochizuki, A. Motomiya, H. Yamagata, and T. Ohta,

Mutagenesis of uracil-DNA glycosylase deficient mutants of the extremely thermophilic eubacterium Thermus thermophilus.

DNA Repair, 7, 663-669 (2008)

 

18. Ohta, T.,

Additive mutagenic effects of DNA damages induced by multiple mutagens at virtually non-mutagenic dose level of each.

Genes Environ., 29, 141-145 (2007)

 

19. Watanabe-Akanuma, M., Y, Inaba, and T. Ohta,

Mutagenicity of UV-irradiated maltol in Salmonella typhimurium.

Mutagenesis, 22, 43-47 (2007)

 

20. Hoogewijs, D., Terwilliger, N, B., Webster, K, A., Powell-Coffman, J, A., Tokishita, S., Yamagata, H., Hankeln, T., Burmester, T., Rytkönen, K, T., Nikinmaa, M., Abele, D., Heise, K., Lucassen, M., Fandrey, J., Maxwell, P, H., Påhlman, S., and Gorr, T, A., 

From critters to cancers: bridging comparative and clinical research on oxygen sensing, HIF signaling, and adaptations towards hypoxia. 

Integr. Comp. Biol., 47, 552-577 (2007)

 

21. Ohta, T.,

Mutagenic activity of a mixture of heterocyclic amines at doses below the biological threshold level of each.

Genes Environ., 28, 181-184 (2006)

 

22. Watanabe-Akanuma, M., Y, Inaba, and T. Ohta,

Analysis of photomutagenicity of thiabendazole with UVA irradiation: Absence of 8-hydroxyguanosine formation.

Genes Environ., 28, 103-107 (2006)

 

23. Ohta, T., S. Tokishita, R. Imazuka, I. Mori, J. Okamura, and H. Yamagata,

β-Glucosidase as a reporter for the gene expression studies in Thermus thermophilus and constitutive expression of DNA repair genes.

Mutagenesis, 21, 255-260 (2006)

 

24. Tokishita, S., Y. Kato, T. Kobayashi, S. Nakamura, T. Ohta, and H. Yamagata,

Organization and repression by juvenile hormone of a vitellogenin gene cluster in the crustacean, Daphnia magna.

Biochem. Biophys. Res. Commun., 345, 362-370 (2006)

 

25. Tokishita, S., S. Kimura, Y. Mandokoro, K. Kato, Y. Shiga, Y. Takahashi, T. Ohta, and H.Yamagata,

Tissue-specific expression of a bHLH-PAS protein homologous to ARNT during the development of crustacean Daphnia magna.

Gene, 376, 231-239  (2006)

 

26. Ohta, T., S. Tokishita, M. Sakahira, K. Mochizuki, J. Kawase, and H. Yamagata,

UV sensitivity and mutagenesis of the extremely thermophilic eubacterium Thermus thermophilus HB27.

Genes Environ., 28, 56-61 (2006)

 

27. Shiga, Y., Sagawa, K., Takai, R., Sakaguchi, H., Yamagata, H., and Hayashi, S.,

Transcriptional readthrough of Hox genes Ubx and Antp and their divergent post-transcriptional control during crustacean evolution.

Evol. Dev., 8, 407-414 (2006)

 

 

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